Isolation of amoebae and Pseudomonas and Legionella spp. from eyewash stations

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Isolation of amoebae and Pseudomonas and Legionella spp. from eyewash stations.

Forty eyewash units were sampled for protozoa, bacteria, and fungi. Total heterotrophic bacterial counts on nutrient agar and R2A agar (Difco Laboratories, Detroit, Mich.) ranged from 0 to 10(5) CFU/ml, with Pseudomonas spp. being the most frequently isolated. Total counts of 10(4) and 10(8) cells per ml were obtained with the acridine orange staining procedure. All samples were examined for Le...

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The development of an amoebal enrichment method, for the recovery of viable Legionella pneumophila from clinical materials is described. The method has been used successfully in five of six cases of Legionnaires' disease in which L pneumophila was isolated. Studies on those L pneumophila isolates led to the discovery that virulent legionellae are attracted to, and attack suitable host amoebae, ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1991

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.57.1.163-167.1991